MCM5 Expression Is Associated With the Grade of Malignancy and Ki-67 Antigen in LSCC

Anticancer Res. 2019 May;39(5):2325-2335. doi: 10.21873/anticanres.13349. Epub 2019 May 15.

Abstract

Background/aim: The minichromosome maintenance proteins (MCMs) may be potential biomarkers of cancer cell proliferation. They are essential to initiate DNA replication. The aim of the study was to investigate the level of MCM5 expression in benign lesions (BLs) and laryngeal squamous cell cancer (LSCC).

Materials and methods: Immunohistochemical (IHC) analysis was carried out on 83 LSCCs and 10 BLs. Western-blot, immunofluorescence analysis (IF) and real-time PCR (RT-PCR) were performed using HEp-2 cancer cells and HaCaT keratinocytes.

Results: The expression of MCM5 was higher in LSCC than in the BLs (p<0.0001) and was higher in subsequent malignancies of LSCC. Positive correlations were demonstrated between the expression levels of MCM5 and the Ki-67 antigen. In vitro studies have confirmed that the expression of MCM5 is elevated in cancer cells.

Conclusion: MCM5 protein may be used as a potential marker of cancer cell proliferation in LSCC.

Keywords: Ki-67; MCM; laryngeal squamous cell cancer; minichromosome proteins.

MeSH terms

  • Aged
  • Biomarkers, Tumor / genetics
  • Cell Cycle Proteins / genetics*
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Keratinocytes / metabolism
  • Ki-67 Antigen / genetics*
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / pathology
  • Male
  • Middle Aged
  • Neoplasms / genetics*
  • Neoplasms / pathology
  • Squamous Cell Carcinoma of Head and Neck / genetics*
  • Squamous Cell Carcinoma of Head and Neck / pathology

Substances

  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • Ki-67 Antigen
  • MCM5 protein, human