Voltammetric Determination of Acetaminophen and Tryptophan Using a Graphite Screen Printed Electrode Modified with Functionalized Graphene Oxide Nanosheets Within a Fe3O4@SiO2 Nanocomposite

Hadi Beitollahi; Fariba Garkani-Nejad; Somayeh Tajik; Mohammad Reza Ganjali

Voltammetric Determination of Acetaminophen and Tryptophan Using a Graphite Screen Printed Electrode Modified with Functionalized Graphene Oxide Nanosheets Within a Fe₃O₄@SiO₂ Nanocomposite

Iran J Pharm Res. 2019 Winter;18(1):80-90.

Authors

Hadi Beitollahi¹, Fariba Garkani-Nejad², Somayeh Tajik³, Mohammad Reza Ganjali^{4

5}

Affiliations

¹ Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
² Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran.
³ NanoBioElectrochemistry Research Center, Bam University of Medical Sciences, Bam, Iran.
⁴ Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran.
⁵ Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 31089346
PMCID: PMC6487427

Abstract

A high sensitive electrochemical nanostructure sensor based on graphene oxide/Fe₃O₄@SiO₂ nanocomposite modified graphite screen printed electrode (GO/Fe₃O₄@SiO₂/SPE) has been developed for trace analysis of acetaminophen. The electrochemical study of the modified electrode, as well as its efficiency for simultaneous voltammetric oxidation of acetaminophen and tryptophan is described. Compared with bare SPE the GO/Fe₃O₄@SiO₂/SPE exhibited excellent electrocatalytic activity toward the oxidation of acetaminophen. The plot of catalytic current versus acetaminophen concentration showed a linear segment in the concentration range 0.5 to 100.0 µM. The detection limit of 0.1 µM was obtained using calibration plot. Also the anodic peaks of acetaminophen and tryptophan in their mixture can be well separated. The GO/Fe₃O₄@SiO₂/SPE has been successfully applied and validated by analyzing acetaminophen and tryptophan in urine and pharmaceutical samples.

Keywords: Acetaminophen; Graphene; Graphite screen printed electrode; Nanocomposite; Tryptophan.