Blimp-1 is involved in B cell activation and maturation in Nile tilapia (Oreochromis niloticus)

Liting Wu; Enxu Zhou; Along Gao; Linghe Kong; Siwei Wu; Xia Bian; Yuan Li; Bingxi Li; Shengli Fu; Zheng Guo; Jianmin Ye

doi:10.1016/j.dci.2019.05.002

Blimp-1 is involved in B cell activation and maturation in Nile tilapia (Oreochromis niloticus)

Dev Comp Immunol. 2019 Sep:98:137-147. doi: 10.1016/j.dci.2019.05.002. Epub 2019 May 10.

Authors

Liting Wu¹, Enxu Zhou¹, Along Gao¹, Linghe Kong¹, Siwei Wu¹, Xia Bian¹, Yuan Li¹, Bingxi Li¹, Shengli Fu¹, Zheng Guo², Jianmin Ye³

Affiliations

¹ School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, Guangdong 510631, PR China.
² School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, Guangdong 510631, PR China. Electronic address: 20151053@m.scnu.edu.cn.
³ School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, Guangdong 510631, PR China. Electronic address: jmye@m.scnu.edu.cn.

PMID: 31078630
DOI: 10.1016/j.dci.2019.05.002

Abstract

B lymphocyte-induced maturation protein 1 (Blimp-1), a transcription factor containing zinc finger, is required and sufficient to trigger terminal differentiation of B cells in mammals. The Blimp-1 (OnBlimp-1) from Nile tilapia (Oreochromis niloticus) was identified and characterized its expression pattern during B cell activation and maturation. The cDNA of OnBlimp-1 open reading frame is 2547 bp encoding a protein of 848 amino acids and the predicted molecular weight is 93.36 kDa. OnBlimp-1 contains a SET domain and five ZnF_C2H2 domains, which shares high homology with that of other species. OnBlimp-1 transcription was detected in all examined tissues with high expression in the spleen (SPL). Analysis of sorted lymphocyte populations, including IgM⁺ and IgM^- cells from peripheral blood (PBL), SPL and anterior kidney (AK), indicated that the OnBlimp-1 transcription was highly expressed in the IgM⁺ B cells. Upon LPS stimulation, OnBlimp-1 expression was up-regulated in tissues of PBL, SPL and AK significantly. The expression of OnBlimp-1, as well as the secreted IgM, was significantly up-regulated in the SPL and AK leukocytes stimulated with anti-OnIgM monoclonal antibody and LPS in vitro, respectively. Above results suggest that OnBlimp-1, a cytokine regulating the terminal differentiation of activated B cells to antibody-secreting cells, is likely to play important roles in B cell activation and maturation in Nile tilapia.

Keywords: B cell activation; Blimp-1; IgM(+) B cell; Oreochromis niloticus; sIgM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
B-Lymphocytes / drug effects
B-Lymphocytes / immunology*
B-Lymphocytes / metabolism
Cell Differentiation / genetics
Cell Differentiation / immunology*
Cichlids / genetics
Cichlids / immunology*
Cichlids / metabolism
Fish Proteins / genetics
Fish Proteins / immunology*
Fish Proteins / metabolism
Gene Expression Profiling / methods
Lipopolysaccharides / immunology
Lipopolysaccharides / pharmacology
Lymphocyte Activation / genetics
Lymphocyte Activation / immunology*
Positive Regulatory Domain I-Binding Factor 1 / genetics
Positive Regulatory Domain I-Binding Factor 1 / immunology*
Positive Regulatory Domain I-Binding Factor 1 / metabolism

Substances

Fish Proteins
Lipopolysaccharides
Positive Regulatory Domain I-Binding Factor 1