A new lateral root growth inhibitor from the sponge-derived fungus Aspergillus sp. LS45

Liming Huang; Lijian Ding; Xiaohui Li; Ning Wang; Yushan Yan; Mengxiang Yang; Wei Cui; C Benjamin Naman; Kejun Cheng; Weiyan Zhang; Bin Zhang; Haixiao Jin; Shan He

doi:10.1016/j.bmcl.2019.04.051

A new lateral root growth inhibitor from the sponge-derived fungus Aspergillus sp. LS45

Bioorg Med Chem Lett. 2019 Jul 1;29(13):1593-1596. doi: 10.1016/j.bmcl.2019.04.051. Epub 2019 Apr 30.

Authors

Liming Huang¹, Lijian Ding², Xiaohui Li¹, Ning Wang¹, Yushan Yan³, Mengxiang Yang³, Wei Cui³, C Benjamin Naman¹, Kejun Cheng⁴, Weiyan Zhang¹, Bin Zhang¹, Haixiao Jin¹, Shan He⁵

Affiliations

¹ Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, 315800 Ningbo, PR China.
² Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, 315800 Ningbo, PR China. Electronic address: dinglijian@nbu.edu.cn.
³ School of Medicine, Ningbo University, 315211 Ningbo, PR China.
⁴ College of Ecology, Lishui University, 323000 Lishui, PR China.
⁵ Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, 315800 Ningbo, PR China. Electronic address: heshan@nbu.edu.cn.

PMID: 31078410
DOI: 10.1016/j.bmcl.2019.04.051

Abstract

Two new γ-lactones, aspergilactones A (1) and B (2), were discovered along with two known compounds, annularin A (3) and pericoterpenoid A (4), from a culture of the sponge-associated fungus Aspergillus sp. LS45. The planar structures of 1-4 were characterized using comprehensive spectroscopic methods and comparison with literature data. The absolute configurations of 1 and 2 were determined by comparison of electronic circular dichroism (ECD) spectroscopic and optical rotation data with those of known analogues as well as calculated ECD analysis. Compounds 1-4 were tested in a variety of bioassays, and both 1 and 4 exhibited significant inhibition against the lateral root growth of Arabidopsis thaliana Columbia-0 at a concentration of 100 μM. In addition, the in vitro cytotoxic activities of 1-4 against six human cancer cell lines CCRF-CEM, K562, BGC823, AGS, HCT-116 and MDA-MB-231 were evaluated. Compound 4 showed moderate inhibitory effects on CCRF-CEM and K562 cancer cell lines with IC₅₀ values of 13.8 ± 1.6 and 12.9 ± 2.5 μM, respectively. However, compounds 1-4 did not show any notable AChE inhibitory activity in vitro.

Keywords: AChE; Aspergillus sp.; Cytotoxicity; Lateral root growth; Sponge-derived fungus; γ-Lactone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus / chemistry*
Fungi / chemistry*
Plant Growth Regulators / chemistry*

Substances

Plant Growth Regulators