Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry

Andrew J Mitchell; Angela Ivask; Yi Ju

doi:10.1007/978-1-4939-9454-0_15

Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry

Methods Mol Biol. 2019:1989:227-241. doi: 10.1007/978-1-4939-9454-0_15.

Authors

Andrew J Mitchell¹, Angela Ivask², Yi Ju³

Affiliations

¹ Materials Characterisation and Fabrication Platform, Department of Chemical Engineering, University of Melbourne, Melbourne, VIC, Australia. andrew.mitchell1@unimelb.edu.au.
² Laboratory of Chemical Physics and Biophysics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
³ Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology and the Department of Chemical Engineering, The University of Melbourne, Parkville, VIC, Australia.

PMID: 31077109
DOI: 10.1007/978-1-4939-9454-0_15

Abstract

Mass cytometry is a technique that uses inductively coupled plasma mass spectrometry (ICP-MS) to quantify the isotopic composition of cells in suspension. Traditionally it has been used in conjunction with antibodies labeled with stable lanthanide isotopes to investigate cellular heterogeneity. Here we describe its use to quantify uptake of metal nanoparticles by cells in suspension.

Keywords: CyTOF; Endocytosis; Inductively coupled plasma mass spectrometry; Mass cytometry; Nanoparticle; Phagocytosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells / cytology
Cells / metabolism*
Flow Cytometry / methods*
Humans
Lanthanoid Series Elements / analysis*
Mass Spectrometry / methods*
Metal Nanoparticles / chemistry*
Metals / analysis*
Single-Cell Analysis / methods*

Substances

Lanthanoid Series Elements
Metals