Bioelectrocatalytic performance of d-fructose dehydrogenase

Taiki Adachi; Yuya Kaida; Yuki Kitazumi; Osamu Shirai; Kenji Kano

doi:10.1016/j.bioelechem.2019.04.024

Bioelectrocatalytic performance of d-fructose dehydrogenase

Bioelectrochemistry. 2019 Oct:129:1-9. doi: 10.1016/j.bioelechem.2019.04.024. Epub 2019 May 2.

Authors

Taiki Adachi¹, Yuya Kaida¹, Yuki Kitazumi¹, Osamu Shirai¹, Kenji Kano²

Affiliations

¹ Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
² Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan. Electronic address: kano.kenji.5z@kyoto-u.ac.jp.

PMID: 31063949
DOI: 10.1016/j.bioelechem.2019.04.024

Abstract

This review summarizes the bioelectrocatalytic properties of d-fructose dehydrogenase (FDH), while taking into consideration its enzymatic characteristics. FDH is a membrane-bound flavohemo-protein with a molecular mass of 138 kDa, and it catalyzes the oxidation of d-fructose to 5-keto-d-fructose. The characteristic feature of FDH is its strong direct-electron-transfer (DET)-type bioelectrocatalytic activity. The pathway of the DET-type reaction is discussed. An overview of the application of FDH-based bioelectrocatalysis to biosensors and biofuel cells is also presented, and the benefits and problems associated with it are extensively discussed.

Keywords: Biofuel cells; Biosensors; Electron transfer pathway; Fructose dehydrogenase; Protein engineering.

Publication types

Review

MeSH terms

Amino Acid Sequence
Bacteria / chemistry
Bacteria / enzymology*
Bacteria / metabolism
Biocatalysis
Bioelectric Energy Sources* / microbiology
Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Carbohydrate Dehydrogenases / chemistry
Carbohydrate Dehydrogenases / metabolism*
Electron Transport
Fructose / metabolism*
Oxidation-Reduction

Substances

Fructose
Carbohydrate Dehydrogenases