Two toll-like receptors identified in the mantle of Mytilus coruscus are abundant in haemocytes

Yi-Feng Li; Yu-Zhu Liu; Yan-Wen Chen; Ke Chen; Frederico M Batista; João C R Cardoso; Yu-Ru Chen; Li-Hua Peng; Ya Zhang; You-Ting Zhu; Xiao Liang; Deborah M Power; Jin-Long Yang

doi:10.1016/j.fsi.2019.05.001

Two toll-like receptors identified in the mantle of Mytilus coruscus are abundant in haemocytes

Fish Shellfish Immunol. 2019 Jul:90:134-140. doi: 10.1016/j.fsi.2019.05.001. Epub 2019 May 2.

Authors

Affiliations

¹ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
² International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.
³ Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, Faro, Portugal.
⁴ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China; Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, Faro, Portugal. Electronic address: dpower@ualg.pt.
⁵ International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: jlyang@shou.edu.cn.

PMID: 31055019
DOI: 10.1016/j.fsi.2019.05.001

Abstract

Toll-like receptors (TLRs) are a large family of pattern recognition receptors (PRRs) that play a critical role in innate immunity. TLRs are activated when they recognize microbial associated molecular patterns (MAMPs) of bacteria, viruses, or fungus. In the present study, two TLRs were isolated from the mantle of the hard-shelled mussel (Mytilus coruscus) and designated McTLR2 and McTLR3 based on their sequence similarity and phylogenetic clustering with Crassostrea gigas, CgiTLR2 and CgiTLR3, respectively. Quantitative RT-PCR analysis demonstrated that McTLR2 and McTLR3 were constitutively expressed in many tissues but at low abundance.

Keywords: Haemocytes; Mantle; Mytilus coruscus; Toll-like receptors.

MeSH terms

Amino Acid Sequence
Animals
Gene Expression Profiling
Hemocytes / immunology*
Hemocytes / metabolism
Immunity, Innate / genetics*
Mytilus / genetics*
Mytilus / immunology*
Phylogeny
Sequence Alignment
Toll-Like Receptors / chemistry
Toll-Like Receptors / genetics*
Toll-Like Receptors / immunology*

Substances

Toll-Like Receptors