Structure of the O-specific polysaccharide of Azospirillum doebereinerae type strain GSF71T

Elena N Sigida; Yuliya P Fedonenko; Alexander S Shashkov; Evelina L Zdorovenko; Svetlana A Konnova; Yuriy A Knirel

doi:10.1016/j.carres.2019.04.009

Structure of the O-specific polysaccharide of Azospirillum doebereinerae type strain GSF71^T

Carbohydr Res. 2019 May 15:478:54-57. doi: 10.1016/j.carres.2019.04.009. Epub 2019 Apr 29.

Authors

Elena N Sigida¹, Yuliya P Fedonenko², Alexander S Shashkov³, Evelina L Zdorovenko³, Svetlana A Konnova⁴, Yuriy A Knirel³

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049, Saratov, Russia. Electronic address: si_elena@mail.ru.
² Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049, Saratov, Russia.
³ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia.
⁴ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049, Saratov, Russia; Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, 410012, Saratov, Russia.

PMID: 31054383
DOI: 10.1016/j.carres.2019.04.009

Abstract

O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of plant-growth-promoting rhizobacteria Azospirillum doebereinerae GSF71^T and studied by sugar analysis along with ¹H and ¹³C NMR spectroscopy, including 2D ¹H,¹H COSY, TOCSY, ROESY, ¹H,¹³C HSQC, and HMBC experiments. It was established that the polysaccharide is linear and consists of tetrasaccharide repeating units with the following structure.

Keywords: Azospirillum doebereinerae; Bacterial polysaccharide structure; Lipopolysaccharide.

MeSH terms

Azospirillum / chemistry*
Carbohydrate Conformation
Hydrolysis
Magnetic Resonance Spectroscopy
Polysaccharides / chemistry*

Substances

Polysaccharides