Molecular Imaging Cellular SPIO Uptake with Nonlinear Optical Microscopy

Lina Machtoub; Rudolf Pfeiffer; Aleksandar Backovic; Stefan Frischauf; Marius C Wick

doi:10.1016/j.jmir.2010.08.001

Molecular Imaging Cellular SPIO Uptake with Nonlinear Optical Microscopy

J Med Imaging Radiat Sci. 2010 Sep;41(3):159-164. doi: 10.1016/j.jmir.2010.08.001. Epub 2011 Jun 8.

Authors

Lina Machtoub¹, Rudolf Pfeiffer², Aleksandar Backovic³, Stefan Frischauf⁴, Marius C Wick⁴

Affiliations

¹ Universitaetsklinik Fuer Radiodiagnostik, Innsbruck Medical University, Innsbruck, Austria. Electronic address: lina.machtoub@i-med.ac.at.
² Faculty of Physics, University of Vienna, Vienna, Austria, and Department of Electrical Engineering, California Institute of Technology, Pasadena.
³ CSO-Research and Development at IDC Immumetrics, Vienna, Austria.
⁴ Universitaetsklinik Fuer Radiodiagnostik, Innsbruck Medical University, Innsbruck, Austria.

PMID: 31051870
DOI: 10.1016/j.jmir.2010.08.001

Abstract

We report a novel application to demonstrate and visualize the selective binding of lipids in cells of the reticuloendothelial system to super paramagnetic iron oxide (SPIO) nanoparticles. Ten New Zealand White rabbits that were experimentally injected intravenously with SPIO and five controls were investigated with vibrational microspectroscopy based on surface-enhanced coherent anti-Stokes Raman scattering (SECARS) microscopy. Marked cellular intensity enhancements in hepatic Kupffer cells and melanomacrophages of spleen have been observed in the range of 2850-2875 cm^-1 in SPIO-injected animals but not in controls. The enhancements are related to the selective association of lipid molecules in cells of the reticuloendothelial system to uptaken SPIO, which can uniquely be visualized with SECARS microscopy.