Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue

Elizabeth D Lightbody; Christopher J B Nicol

doi:10.1007/978-1-4939-9195-2_8

Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue

Methods Mol Biol. 2019:1966:101-105. doi: 10.1007/978-1-4939-9195-2_8.

Authors

Elizabeth D Lightbody^{1

2}, Christopher J B Nicol^{3

4

5}

Affiliations

¹ Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.
² Division of Cancer Biology and Genetics, Cancer Research Institute, Queen's University, Kingston, ON, Canada.
³ Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada. nicolc@queensu.ca.
⁴ Division of Cancer Biology and Genetics, Cancer Research Institute, Queen's University, Kingston, ON, Canada. nicolc@queensu.ca.
⁵ Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. nicolc@queensu.ca.

PMID: 31041741
DOI: 10.1007/978-1-4939-9195-2_8

Abstract

Immunofluorescent staining (IF) uses antigen-antibody complexes tagged with fluorochromes to observe the expression of proteins within a tissue sample. Multiple groups have described optimized methods to visualize several proteins simultaneously within the same tissue section using immunofluorescence in both mouse and human FFPE tissues. Our group routinely uses an optimized protocol described here to examine nuclear receptor expression in experimental samples from conditional knockout in vivo studies.

Keywords: FFPE; Immunofluorescent labeling; Nuclear receptor.

MeSH terms

Animals
Fluorescent Antibody Technique / methods*
Formaldehyde
Gene Expression*
Humans
Mice
Paraffin Embedding*
Receptors, Cytoplasmic and Nuclear / analysis*
Receptors, Cytoplasmic and Nuclear / genetics

Substances

Receptors, Cytoplasmic and Nuclear
Formaldehyde