Analysis of tRNACys processing in the absence of CCAase in Bacillus subtilis

Braz J Microbiol. 2019 Jul;50(3):613-618. doi: 10.1007/s42770-019-00075-5. Epub 2019 Apr 12.

Abstract

In Bacillus subtilis, the tRNACys lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNACys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNACys.

Keywords: Bacillus; CCAase; tRNACys.

MeSH terms

  • Bacillus subtilis / chemistry
  • Bacillus subtilis / enzymology*
  • Bacillus subtilis / genetics
  • Bacillus subtilis / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Nucleic Acid Conformation
  • RNA Nucleotidyltransferases / deficiency*
  • RNA Nucleotidyltransferases / genetics
  • RNA Processing, Post-Transcriptional
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / genetics*
  • RNA, Bacterial / metabolism
  • RNA, Transfer, Cys / chemistry
  • RNA, Transfer, Cys / genetics*
  • RNA, Transfer, Cys / metabolism

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • RNA, Transfer, Cys
  • RNA Nucleotidyltransferases
  • tRNA nucleotidyltransferase