Abstract
In Bacillus subtilis, the tRNACys lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNACys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNACys.
Keywords:
Bacillus; CCAase; tRNACys.
MeSH terms
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Bacillus subtilis / chemistry
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Bacillus subtilis / enzymology*
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Bacillus subtilis / genetics
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Bacillus subtilis / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence
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Nucleic Acid Conformation
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RNA Nucleotidyltransferases / deficiency*
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RNA Nucleotidyltransferases / genetics
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RNA Processing, Post-Transcriptional
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RNA, Bacterial / chemistry
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RNA, Bacterial / genetics*
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RNA, Bacterial / metabolism
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RNA, Transfer, Cys / chemistry
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RNA, Transfer, Cys / genetics*
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RNA, Transfer, Cys / metabolism
Substances
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Bacterial Proteins
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RNA, Bacterial
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RNA, Transfer, Cys
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RNA Nucleotidyltransferases
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tRNA nucleotidyltransferase