In the present investigation, extremely fine Hydroxyapatite (HAP) powder was prepared through chemical precipitation technique under the influence of a nonionic surfactant (Brij 35-Polyoxyethylene lauryl ether). The samples were sintered at 800 °C to obtain highly pure HAP phase. The functional group present in the HAP powder was confirmed by using Fourier transform infra-red (FTIR) spectroscopy. The change in Phase transformation, the crystallite size and the percentage of crystallinity of the synthesized sample were studied by X-ray diffractometer technique. The Field Emission Scanning Electron Microscope (FESEM) and High-resolution transmission electron microscopy (HRTEM) were used for the structural analysis of porous rod-shaped HAP crystal. Further, drug loading and In Vitro leaching kinetics were conducted for antibiotics-ciproflaxin (CPF). Antimicrobial activity against S. aureus (Gram-positive bacterium) and E. coli (Gram-negative bacterium) was executed for pure HAP as well as for drug loaded HAP.