Water dispersible graphene layer are the excellent nano materials used for wide range of electronic applications. High quality graphene was synthesized by an eco-friendly, easy and cost effective electrochemical exfoliation method. In this work, graphite rod was used both as an anode and cathode for the production of graphene. Potassium sulphate (K₂SO₄) was used as an intercalating agent. Electrochemically exfoliated graphene (EEG) was coated on glassy carbon electrode (GCE) and evaluated towards the electrochemical oxidation of vanillin and L-phenylalanine. The fabricated electrode was able to detect vanillin and L-Phenylalanine as low as 0.2 μM with signal to noise ratio of 3. A significant increase in the current was observed for the graphene coated electrode for both vanillin and L-phenylalanine when compared to bare Glassy electrode. The finding clearly demonstrated the higher detection capability, selectivity and reproducibility of EEG.