CTX-M-15-producing uropathogenic Escherichia coli isolates at Rio de Janeiro, Brazil: Molecular epidemiology and MALDI-TOF MS

New Microbiol. 2019 Apr;42(2):121-124. Epub 2019 Apr 29.

Abstract

Because of the high prevalence of CTX-M-15-producing Escherichia coli isolates causing urinary tract infections in Rio de Janeiro, we have investigated bla-CTX-M-15 gene presence, as well as CTX-M-15 production, in 32 E. coli isolates recovered from the urine of outpatients assisted at a public hospital located in the west zone of Rio. Molecular epidemiology was assessed by PFGE and phylo-typing methods. The work highlights the good performance of MALDI-TOF MS as an alternative tool to detect extended-spectrum beta-lactamases among CTX-M-15-producing E. coli isolates.

Keywords: CTX-M-15; Escherichia coli; MALDI-TOF MS; molecular typing.

MeSH terms

  • Brazil / epidemiology
  • Electrophoresis, Gel, Pulsed-Field
  • Escherichia coli Infections* / epidemiology
  • Escherichia coli Infections* / microbiology
  • Humans
  • Molecular Epidemiology*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Urinary Tract Infections / epidemiology
  • Urinary Tract Infections / microbiology
  • Urine / microbiology
  • Uropathogenic Escherichia coli* / chemistry
  • Uropathogenic Escherichia coli* / classification
  • Uropathogenic Escherichia coli* / enzymology
  • Uropathogenic Escherichia coli* / genetics
  • beta-Lactamases* / genetics

Substances

  • beta-Lactamases