Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

Biol Trace Elem Res. 2020 Feb;193(2):410-413. doi: 10.1007/s12011-019-01728-0. Epub 2019 Apr 26.

Abstract

This report explains the employing of a combination test of traditional cell culture with a quantitative real-time PCR for assessment of the antiviral effect of zinc sulfate (ZnSO4) on herpes simplex virus (HSV)-infected Vero cells. Our evidence showed that the treatment with 0.3 mM ZnSO4 strongly inhibited the replication of virus progeny (MOI 0.001) at least 68-fold less. On the other hand, the IC50 demonstrated that the highest activity of ZnSO4 was at the 0.23 mM concentration.

Keywords: Antiviral effect; HSV; Herpes simplex virus; Zinc sulfate.

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology
  • Cell Survival / drug effects
  • Chlorocebus aethiops
  • Cytopathogenic Effect, Viral / drug effects
  • DNA Replication / drug effects
  • DNA Replication / genetics
  • Herpesvirus 1, Human / drug effects*
  • Herpesvirus 1, Human / genetics
  • Herpesvirus 1, Human / physiology
  • Inhibitory Concentration 50
  • Vero Cells
  • Virus Replication / drug effects*
  • Virus Replication / genetics
  • Zinc Sulfate / pharmacology*

Substances

  • Antiviral Agents
  • Zinc Sulfate