The protein elicitor Hrip1 enhances resistance to insects and early bolting and flowering in Arabidopsis thaliana

PLoS One. 2019 Apr 25;14(4):e0216082. doi: 10.1371/journal.pone.0216082. eCollection 2019.

Abstract

The elicitor Hrip1 isolated from necrotrophic fungus Alternaria tenuissima, could induce systemic acquired resistance in tobacco to enhance resistance to tobacco mosaic virus. In the present study, we found that the transgenic lines of Hrip1-overexpression in wild type (WT) Arabidopsis thaliana were more resistant to Spodoptera exigua and were early bolting and flowering than the WT. A profiling of transcription assay using digital gene expression profiling was used for transgenic and WT Arabidopsis thaliana. Differentially expressed genes including 40 upregulated and three downregulated genes were identified. In transgenic lines of Hrip1-overexpression, three genes related to jasmonate (JA) biosynthesis were significantly upregulated, and the JA level was found to be higher than WT. Two GDSL family members (GLIP1 and GLIP4) and pathogen-related gene, which participated in pathogen defense action, were upregulated in the transgenic line of Hrip1-overexpression. Thus, Hrip1 is involved in affecting the flower bolting time and regulating endogenous JA biosynthesis and regulatory network to enhance resistance to insect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis / genetics*
  • Arabidopsis / immunology
  • Arabidopsis / parasitology*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cyclopentanes / metabolism
  • Disease Resistance*
  • Flowers / physiology*
  • Gene Expression Regulation, Plant
  • Oxylipins / metabolism
  • Photoperiod
  • Plant Diseases / immunology*
  • Plant Diseases / parasitology*
  • Plants, Genetically Modified
  • Reproducibility of Results
  • Spodoptera / physiology*

Substances

  • Arabidopsis Proteins
  • Cyclopentanes
  • Oxylipins
  • jasmonic acid

Grants and funding

This work supported by grant from the National Natural Science Foundation of China (No. 31301651) and grants from Scientific Research Common Program of Beijing Municipal Commission of Education (SQKM201710011010), both to ZC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.