Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA

Petra Ménová; Hana Cahová; Milan Vrábel; Michal Hocek

doi:10.1007/978-1-4939-9216-4_4

Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA

Methods Mol Biol. 2019:1973:39-57. doi: 10.1007/978-1-4939-9216-4_4.

Authors

Petra Ménová¹, Hana Cahová¹, Milan Vrábel¹, Michal Hocek²

Affiliations

¹ Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.
² Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic. hocek@uochb.cas.cz.

PMID: 31016695
DOI: 10.1007/978-1-4939-9216-4_4

Abstract

Synthesis of base-modified dNTPs through the Suzuki or Sonogashira cross-coupling reactions of halogenated dNTPs with boronic acids or alkynes is reported, as well as the use of these modified dNTPs in polymerase incorporations to oligonucleotides or DNA by primer extension or PCR.

Keywords: DNA polymerases; Enzymatic synthesis; Modified DNA; Nucleoside triphosphates; Nucleotides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / biosynthesis*
DNA / chemistry
DNA-Directed DNA Polymerase / metabolism*
Halogenation
Nucleotides / biosynthesis*
Nucleotides / chemistry

Substances

Nucleotides
DNA
DNA-Directed DNA Polymerase