Chickens are a host that is very resistant to the development of clinical toxoplasmosis. Free-range chickens have been used to indirectly track environmental contamination with Toxoplasma gondii oocysts because they feed on the ground. This study evaluated the genetic diversity of T. gondii isolates from free-range chickens from Florianópolis island in Santa Catarina state, southern Brazil. Sera from 21 chickens were tested for IgG anti-T. gondii antibodies using the modified agglutination test (MAT). Tissue homogenates from the 11 seropositive birds (MAT titres ≥5) were bioassayed in mice. The four obtained isolates (TgCkBrSC1-4) were genotyped using 11 PCR-RFLP markers and 15 microsatellite markers (MS). Four genotypes were identified, three of which are typical Brazilian genotypes (ToxoDB-RFLP #26 and #53 were previously reported and #278 is new), and the other is the rare clonal type I genotype. This type I isolate was considered a variant according to MS analysis, with two atypical alleles, which emphasizes the genetic diversity of the parasite in Brazil. The genetic variability of T. gondii in South America may be related to the high occurrence of severe ocular and congenital toxoplasmosis in humans in this region. High human seroprevalence and frequency of ocular toxoplasmosis are reported in southern Brazil, but there is limited information on the T. gondii strains that are circulating in this region, so more studies should be conducted to identify the strains in different hosts and in human toxoplasmosis cases.
Keywords: Bioassay; Gallus Gallus domesticus; Genotyping; Isolation; Toxoplasmosis.
Copyright © 2018 Elsevier B.V. All rights reserved.