Training-induced changes on quadriceps muscle oxygenation measured by near-infrared spectroscopy in healthy subjects and in chronic obstructive pulmonary disease patients

Abstract

Aim: We hypothesize that training-induced changes in muscle oxygen saturation (StO₂ ) assessed by near-infrared spectroscopy (NIRS) during constant work rate cycling exercise (CWRE) may be a useful marker of the effects of training at 'vastus medialis' of the quadriceps in patients with chronic obstructive pulmonary disease (COPD).

Methods: Incremental exercise [peak oxygen uptake (VO₂ )] and CWRE at 70% pretraining peak VO₂ , before and after 8-w training, were done in 10 healthy age-matched subjects (H) [80% men, 65(11) years, FEV₁ 105(14)%] and 16 COPD patients [94% men, 70(5) years, FEV₁ 46(11) %] encompassing the entire spectrum of disease severity, recruited in the outpatient clinics. NIRS was used to assess StO₂ in the 'vastus medialis' of the left quadriceps.

Results: Pretraining CWRE decreased StO₂ (P<0·05) and generated marked StO₂ rebound (P<0·001) after unloading in the two groups. After training, VO₂ peak increased in H [253(204) ml min^-1 ] (P<0·01) and in COPD [180(183) ml·min^-1 ] (P = 0·01) and blood lactate fell [-4·4 (2·7) and -1·6(2·3) mmol·m^-1 ] (P<0·05 each). Training generated a further fall in StO₂ during CWRE [-10(12)% and -10(10)%, P<0·05] and increased StO₂ rebound after unloading [8(7)% and 5(9)%, P<0·05] in both groups.

Conclusion: Endurance training further decreased StO₂ during CWRE, similarly in both groups, likely due to training-induced enhancement of muscle O₂ transfer and utilization. Training-induced StO₂ fall during CWRE may be useful individual marker for non-invasive assessment of enhanced muscle aerobic post-training function.

Keywords: exercise testing; exercise training; muscle oxygen saturation; photonics.