The New Plant Expression System for the Development of Vaccines against Papillomaviruses

Dokl Biochem Biophys. 2019 May;484(1):52-54. doi: 10.1134/S1607672919010150. Epub 2019 Apr 22.

Abstract

To enhance the synthesis of antigenic envelope proteins L1 of high-grade papillomavirus types HPV16, HPV18, HPV31, and HPV45, the sequence of the gene encoding the cucumber mosaic virus replicase (RdRP CMV) was inserted into the genetic construct. This made it possible to increase the production of these antigenic proteins to 25-27 μg/mg total soluble protein.

MeSH terms

  • Cucumovirus* / genetics
  • Cucumovirus* / metabolism
  • Epitopes* / biosynthesis
  • Epitopes* / chemistry
  • Epitopes* / genetics
  • Papillomaviridae / genetics*
  • Papillomavirus Vaccines* / biosynthesis
  • Papillomavirus Vaccines* / chemistry
  • Papillomavirus Vaccines* / genetics
  • Solanum lycopersicum* / chemistry
  • Solanum lycopersicum* / genetics
  • Solanum lycopersicum* / metabolism
  • Solanum lycopersicum* / virology
  • Viral Proteins* / biosynthesis
  • Viral Proteins* / chemistry
  • Viral Proteins* / genetics

Substances

  • Epitopes
  • Papillomavirus Vaccines
  • Viral Proteins