Comparison between Roche Integra 400 plus and Abbott Architect ci8200 in Alanine aminotransferase assay

Somiya Lamrabat; Amina Lyagoubi; Ouardia Bouayadi; Jalila Elmalki; Khadija Elkouche; Mohammed Bensalah; Mohammed Choukri

doi:10.1016/j.plabm.2019.e00121

Comparison between Roche Integra 400 plus and Abbott Architect ci8200 in Alanine aminotransferase assay

Pract Lab Med. 2019 Mar 22:15:e00121. doi: 10.1016/j.plabm.2019.e00121. eCollection 2019 May.

Authors

Somiya Lamrabat^{1

2}, Amina Lyagoubi^{1

2}, Ouardia Bouayadi^{1

2}, Jalila Elmalki^{1

2}, Khadija Elkouche^{1

2}, Mohammed Bensalah^{1

2}, Mohammed Choukri^{1

2}

Affiliations

¹ Central Laboratory of Mohammed VI University Hospital of Oujda City, Morocco.
² Faculty of Medicine and Pharmacy, First Mohammed University, Oujda City, Morocco.

Abstract

Background: The aim of this work is to present the results of a comparative study between the ALT assay on Integra 400 plus Roche Diagnostic versus Architect ci8200 of Abbott Diagnostic.

Methods: A total of 200 patients hospitalized in the various departments of the university hospital Mohammed VI of Oujda were prospectively tested on two systems: Abbott Architect ci8200 and Roche Integra 400 plus. Both analyzers use the spectrophotometric technique by coupling the transamination reaction to the oxidation-reduction reaction at NAD. The agreement of the results between the different techniques was evaluated using the Bland-Altman difference diagram and the Passing-Bablok and Deming regression line.

Results: There was a high concordance between the two assays: the equation of the Passing-Bablok line is YArchitect = -0,5625 + 0,9917 XIntegra with a correlation coefficient r² = 0.999. The Bland-Altman diagram shows that the mean bias between the two methods is of the order of 1.1 IU/L and the difference between the ALT measurements by Architect and Integra is in the range of -1.4 to 3.6.

Conclusion: Our study shows a high correlation of the ALT assay results between the architect ci8200 and Integra 400 plus.

Keywords: Abbott diagnostic; Alanine aminotransferase; Comparison; Concordance; Roche diagnostic.