Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of H2O2 Released from Live Cells

Danting Yang; Ning Ni; Lu Cao; Xin Song; Yasmin Alhamoud; Guangxia Yu; Jinshun Zhao; Haibo Zhou

doi:10.3390/mi10040268

Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of H₂O₂ Released from Live Cells

Micromachines (Basel). 2019 Apr 21;10(4):268. doi: 10.3390/mi10040268.

Authors

Danting Yang¹, Ning Ni², Lu Cao³, Xin Song⁴, Yasmin Alhamoud⁵, Guangxia Yu⁶, Jinshun Zhao⁷, Haibo Zhou⁸

Affiliations

¹ Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China. yangdanting@nbu.edu.cn.
² Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China. nn15058476109@gmail.com.
³ School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. caolu5@mail2.sysu.edu.cn.
⁴ Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China. 1611101232@nbu.edu.cn.
⁵ Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China. yasmeenfood@hotmail.com.
⁶ Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China. yuguangxia@nbu.edu.cn.
⁷ Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo 315211, China. zhaojinshun@nbu.edu.cn.
⁸ Institute of Pharmaceutical Analysis and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China. haibo.zhou@jnu.edu.cn.

Abstract

In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (H₂O₂) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of H₂O₂ concentration variation at an optimized potential of -0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO₂ nanoparticles/glass carbon electrode (Ag-mSiO₂ NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine H₂O₂ released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics.

Keywords: electrochemical sensor; hydrogen peroxide; live cells; silver-mesoporous silica nanoparticles.

Abstract

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