Pulsed electric field: Effect on in-vitro simulated gastrointestinal protein digestion of deer Longissimus dorsi

Zuhaib F Bhat; James D Morton; Susan L Mason; Alaa El-Din A Bekhit; Tanyaradzwa E Mungure

doi:10.1016/j.foodres.2018.11.040

Pulsed electric field: Effect on in-vitro simulated gastrointestinal protein digestion of deer Longissimus dorsi

Food Res Int. 2019 Jun:120:793-799. doi: 10.1016/j.foodres.2018.11.040. Epub 2018 Nov 19.

Authors

Zuhaib F Bhat¹, James D Morton², Susan L Mason³, Alaa El-Din A Bekhit⁴, Tanyaradzwa E Mungure⁵

Affiliations

¹ Department of Wine Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, 7647 Christchurch, New Zealand. Electronic address: Zuhaib.Bhat@lincolnuni.ac.nz.
² Department of Wine Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, 7647 Christchurch, New Zealand. Electronic address: James.Morton@lincolnuni.ac.nz.
³ Department of Wine Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, 7647 Christchurch, New Zealand. Electronic address: Sue.Mason@lincoln.ac.nz.
⁴ Department of Food Sciences, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand.
⁵ Department of Food Sciences, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand. Electronic address: aladin.bekhit@otago.ac.nz.

PMID: 31000299
DOI: 10.1016/j.foodres.2018.11.040

Abstract

The effect of pulsed electric field (PEF) on in-vitro simulated gastrointestinal protein digestion of cold-boned deer Longissimus dorsi was elucidated. PEF treated samples viz. T₁ (2.5 kV, 50 Hz) and T₂ (10 kV, 90 Hz) along with a control were subjected to in-vitro simulated gastrointestinal protein digestion. Samples were collected after 0, 30, and 60 min of gastric digestion and 120 and 180 min of intestinal digestion. A significant (P < 0.05) increase was observed in the protein digestibility and soluble protein (%) of PEF treated samples, highlighting a positive influence of PEF processing on the digestion process. Higher concentrations of almost all the free amino acids were observed for the PEF treated samples. No significant effect of PEF was observed on the release of various minerals. PEF may be utilized for the development of novel muscle foods with improved protein digestibility.

Keywords: Digestibility (%); Free amino acid analysis; In-vitro protein digestion; Longissimus dorsi; Mineral profile; Pulsed electric field; Soluble protein (%); Venison.

MeSH terms

Animals
Deer / metabolism*
Digestion*
Food Handling / methods*
Gastrointestinal Tract
In Vitro Techniques
Paraspinal Muscles / metabolism
Physical Phenomena
Red Meat / analysis*