Reduction of β-ODAP and IP6 contents in Lathyrus sativus L. seed by high hydrostatic pressure

Meseret Bekele Buta; Shimelis Admassu Emire; Clemens Posten; Sabine Andrée; Ralf Greiner

doi:10.1016/j.foodres.2019.02.011

Reduction of β-ODAP and IP₆ contents in Lathyrus sativus L. seed by high hydrostatic pressure

Food Res Int. 2019 Jun:120:73-82. doi: 10.1016/j.foodres.2019.02.011. Epub 2019 Feb 14.

Authors

Meseret Bekele Buta¹, Shimelis Admassu Emire², Clemens Posten³, Sabine Andrée⁴, Ralf Greiner⁵

Affiliations

¹ School of Chemical and Bioengineering, Department of Food Engineering, Addis Ababa Institute of Technology, P.O.B: 1176, Addis Ababa, Ethiopia; Department of Food Technology and Bioprocess Engineering, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Haid-und-Neu-Str. 9, 76131 Karlsruhe, Germany; Institute of Process Engineering in Life Sciences III Bioprocess Engineering, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany.
² School of Chemical and Bioengineering, Department of Food Engineering, Addis Ababa Institute of Technology, P.O.B: 1176, Addis Ababa, Ethiopia.
³ Institute of Process Engineering in Life Sciences III Bioprocess Engineering, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany.
⁴ Institute for Safety and Quality of Meat, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, E.-C.-Baumann-Straße 20, 95326 Kulmbach, Germany.
⁵ Department of Food Technology and Bioprocess Engineering, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Haid-und-Neu-Str. 9, 76131 Karlsruhe, Germany. Electronic address: ralf.greiner@mri.bund.de.

PMID: 31000291
DOI: 10.1016/j.foodres.2019.02.011

Abstract

Grass pea (Lathyrus sativus L.) seeds contain an endogenous neurotoxic non-proteinogenic amino acid, β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP), a major limiting factor-for their human consumption. Furthermore, phytate (IP₆), a well-known antinutrient is present in concentration capable of hindering bioavailability of iron (Fe), zinc (Zn), calcium (Ca), phosphorus (P) and other micronutrients from the seeds. Due to the reported capability of high hydrostatic pressure (HHP) to reduce the content of certain antinutritional/toxic agents in seeds and grains, the impact of HHP on the reduction of β-ODAP and IP₆ were investigated. The contents of β-ODAP of accessions from different regions in Ethiopia were found to be in the range of 51.94 to 806.52 mg/100 g. Accession (GF₁- Alemu, AK) exhibiting the highest β-ODAP content was selected for HHP treatment in soaked and batter forms using Central Composite Face Centered Design of experiments. The best HHP conditions in respect to β-ODAP reduction were also applied to the accession (GP-240038) with the lowest β-ODAP-content, a genetically improved variety (Wassie) and a variety from Germany (GR). The HHP treatment at 600 MPa for 25 min of seeds soaked for 6 h and 12 h exhibited the maximum reduction of β-ODAP (232.11 mg/100 g) and IP₆ (21.11 mg/100 g) respectively. The combined incremental effect of pressure and soaking time resulted in a more significant (p ≤ .001) reduction in both compounds than the interaction of pressure with holding time (p ≤ .05). A reduction of β-ODAP from 36.00 to 71.22% by soaked-HHP treatment was observed. β-ODAP reductions were always higher for soaked compared to batter grass pea seeds. IP₆ contents after HHP treatment ranged from 33.65 mg/100 g to nill. It can be concluded that pressure, soaking and holding time as well as the grass pea seed accession/variety had great impact on molecular structure changes, enhancement of enzyme activity and reduction in β-ODAP and IP₆ content.

Keywords: Accession; Batter; HHP; IP(6); Lathyrus sativus L.; Proteinogenic amino acids; Soaking; Variety; β-ODAP.

MeSH terms

Amino Acids, Diamino / analysis*
Biological Availability
Food Handling / methods*
Humans
Hydrostatic Pressure*
Lathyrus / chemistry*
Micronutrients
Phytic Acid / analysis*
Seeds / chemistry*
Water
beta-Alanine / analogs & derivatives*
beta-Alanine / analysis

Substances

Amino Acids, Diamino
Micronutrients
Water
beta-Alanine
oxalyldiaminopropionic acid
Phytic Acid
2,3-diaminopropionic acid