Two lytic transglycosylases of Xanthomonas campestris pv. campestris associated with cell separation and type III secretion system, respectively

FEMS Microbiol Lett. 2019 Apr 1;366(7):fnz073. doi: 10.1093/femsle/fnz073.

Abstract

The lytic transglycosylases (LTs) are important enzymes that degrade peptidoglycan of the bacterial cell wall and affect many biological functions. We present here that XC_0706 and XC_3001 are annotated as the LTs in Xanthomonas campestris pv. campestris. XC_0706 is associated with virulence and plays a pivotal role in cell division. Mutation on XC_3001 reduced hypersensitive response induction and the translocation of type III effector, but did not affect the function of the type II secretion system. Further studies showed that multiple LTs genes contribute to efficiency of the type III secretory system in X. campestris pv. campestris.

Keywords: Xanthomonas; cell separation; hypersensitive response; lytic transglycosylases; virulence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Capsicum / microbiology
  • Gene Expression Regulation, Bacterial
  • Glycosyltransferases / genetics
  • Glycosyltransferases / metabolism*
  • Plant Diseases / microbiology
  • Type III Secretion Systems / genetics
  • Type III Secretion Systems / metabolism*
  • Virulence
  • Xanthomonas campestris / enzymology*
  • Xanthomonas campestris / genetics
  • Xanthomonas campestris / physiology

Substances

  • Bacterial Proteins
  • Type III Secretion Systems
  • Glycosyltransferases