Electrochemical fabrication of fibrin gels via cascade reaction for cell culture

Noriko Taira; Kosuke Ino; Tatsuki Kumagai; Yuji Nashimoto; Hitoshi Shiku

doi:10.1039/c9cc01576k

Electrochemical fabrication of fibrin gels via cascade reaction for cell culture

Chem Commun (Camb). 2019 May 10;55(37):5335-5338. doi: 10.1039/c9cc01576k. Epub 2019 Apr 10.

Authors

Noriko Taira¹, Kosuke Ino¹, Tatsuki Kumagai², Yuji Nashimoto³, Hitoshi Shiku¹

Affiliations

¹ Graduate School of Engineering, Tohoku University, 6-6-11 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan. kosuke.ino@tohoku.ac.jp hitoshi.shiku.c3@tohoku.ac.jp.
² Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
³ Graduate School of Engineering, Tohoku University, 6-6-11 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan. kosuke.ino@tohoku.ac.jp hitoshi.shiku.c3@tohoku.ac.jp and Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6-3 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8578, Japan.

PMID: 30968103
DOI: 10.1039/c9cc01576k

Abstract

We present a new strategy for fabricating fibrin gels by electrochemically controlling a cascade reaction and its application in cell culture. In this strategy, Ca²⁺ is released from CaCO₃ particles via electrochemical acidification. In the presence of the released Ca²⁺, the activated cascade reaction produces fibrin gels.

MeSH terms

Calcium / chemistry
Calcium / metabolism
Calcium Carbonate / chemistry*
Cell Line
Electrochemical Techniques
Fibrin / chemistry*
Fibrin / metabolism
Fibrinogen / metabolism
Fibroblasts / cytology
Fibroblasts / metabolism
Gels / chemistry*
Humans
Hydrogels / chemistry
Prothrombin / metabolism

Substances

Gels
Hydrogels
Prothrombin
Fibrin
Fibrinogen
Calcium Carbonate
Calcium