Preparation of Molecularly Imprinted Mesoporous Materials for Highly Enhancing Adsorption Performance of Cytochrome C

Zhiling Li; Ping Guan; Xiaoling Hu; Shichao Ding; Yuan Tian; Yarong Xu; Liwei Qian

doi:10.3390/polym10030298

Preparation of Molecularly Imprinted Mesoporous Materials for Highly Enhancing Adsorption Performance of Cytochrome C

Polymers (Basel). 2018 Mar 10;10(3):298. doi: 10.3390/polym10030298.

Authors

Zhiling Li¹, Ping Guan², Xiaoling Hu³, Shichao Ding⁴, Yuan Tian⁵, Yarong Xu⁶, Liwei Qian⁷

Affiliations

¹ Key Laboratory of Space Applied Physics and Chemistry of Ministry of Education, Department of Applied Chemistry, School of Nature and Applied Science, Northwestern Polytechnical University, Xi'an 710072, China. lizhiling1993@mail.nwpu.edu.cn.
² Key Laboratory of Space Applied Physics and Chemistry of Ministry of Education, Department of Applied Chemistry, School of Nature and Applied Science, Northwestern Polytechnical University, Xi'an 710072, China. guanping1113@nwpu.edu.cn.
³ Key Laboratory of Space Applied Physics and Chemistry of Ministry of Education, Department of Applied Chemistry, School of Nature and Applied Science, Northwestern Polytechnical University, Xi'an 710072, China. huxl@nwpu.edu.cn.
⁴ Key Laboratory of Space Applied Physics and Chemistry of Ministry of Education, Department of Applied Chemistry, School of Nature and Applied Science, Northwestern Polytechnical University, Xi'an 710072, China. dingshichao1992@163.com.
⁵ Key Laboratory of Space Applied Physics and Chemistry of Ministry of Education, Department of Applied Chemistry, School of Nature and Applied Science, Northwestern Polytechnical University, Xi'an 710072, China. summer940324@163.com.
⁶ Key Laboratory of Space Applied Physics and Chemistry of Ministry of Education, Department of Applied Chemistry, School of Nature and Applied Science, Northwestern Polytechnical University, Xi'an 710072, China. xuyarong@mail.nwpu.edu.cn.
⁷ College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. qianliwei@mail.nwpu.edu.cn.

Abstract

Molecularly imprinted mesoporous materials (MIMs) were synthesized to improve the adsorption performance of Cytochrome c (Cyt c) by using an imidazolium-based amphiphilic ionic liquid 1-octadecyl-3-methylimidazolium chloride (C₁₈MIMCl) as surfactant in aqueous solution via the epitope imprinting approach. The surface-exposed C-terminus nonapeptide of Cyt c (residues 96⁻104, AYLKKATNE) was utilized as the imprinted template. The nitrogen adsorption-desorption, thermo-gravimetric analysis, and transmission electron microscopy verified the successful preparation of MIMs with ordered mesoporous structure. The adsorption isotherm studies showed that the obtained MIMs exhibited superior adsorption capacity toward Cyt c of 86.47 mg·g^-1 because of the high specific surface areas of 824 m²·g^-1, and the appropriate pore size promoted the mass transfer of Cyt c, causing a rapid adsorption equilibrium within 20 min. Furthermore, these MIMs still remained excellent selectivity and recognition ability according to the selective as well as the competitive adsorption studies, suggesting that the molecularly imprinted mesoporous materials is expected to be used in the field of highly efficient separation and enrichment of proteins.

Keywords: Cytochrome c; amphiphilic ionic liquids; epitope imprinting; molecularly imprinted mesoporous silica.