LncRNA CASC11 promotes TGF-β1, increases cancer cell stemness and predicts postoperative survival in small cell lung cancer

Ying Fu; Pu Zhang; Hui Nan; Yiming Lu; Jun Zhao; Mengxiang Yang; Qingcui Song

doi:10.1016/j.gene.2019.04.019

LncRNA CASC11 promotes TGF-β1, increases cancer cell stemness and predicts postoperative survival in small cell lung cancer

Gene. 2019 Jul 1:704:91-96. doi: 10.1016/j.gene.2019.04.019. Epub 2019 Apr 6.

Authors

Ying Fu¹, Pu Zhang¹, Hui Nan², Yiming Lu³, Jun Zhao¹, Mengxiang Yang¹, Qingcui Song⁴

Affiliations

¹ Department of Oncology, Liaocheng People's Hospital, Liaocheng City, Shandong Province 252000, PR China.
² Department of Children Healthcare, Liaocheng People's Hospital, Liaocheng City, Shandong Province 252000, PR China.
³ Department of Information, Liaocheng People's Hospital, Liaocheng City, Shandong Province 252000, PR China.
⁴ Department of Oncology, Liaocheng People's Hospital, Liaocheng City, Shandong Province 252000, PR China. Electronic address: jiycdz9@163.com.

PMID: 30965130
DOI: 10.1016/j.gene.2019.04.019

Abstract

LncRNA CASC11 is a recently identified oncogenic lncRNA in colorectal cancer. This study aimed to investigate the role of lncRNA CASC11 in small cell lung cancer (SCLC). In the present study, expression levels of CASC11 and TGF-β1 were found to be positively and significantly correlated with the percentage of CDD133+ cells of SCLC cell lines. Plasma CASC11 and TGF-β1 were upregulated and positively correlated in SCLC patients, but not in healthy controls. Upregulation of plasma CASC11 and TGF-β1 predicted poor survival of SCLC patients. Overexpression of CASC11 and TGF-β1 also resulted in the increased percentage of CDD133+ cells of SCLC cell lines, while TGF-β inhibitor attenuated the effects of CASC11 overexpression. CASC11 overexpression mediated the upregulation of TGF-β1 in SCLC cells, while treatment with exogenous TGF-β1 showed no significant effect on CASC11. Therefore, lncRNA CASC11 promotes TGF-β1, increases cancer cell stemness and predicts postoperative survival in SCLC.

Keywords: LncRNA CASC11; Small cell lung cancer; Stemness; Survival; TGF-β1.

MeSH terms

Adult
Aged
Biomarkers, Tumor / physiology
Case-Control Studies
Cell Proliferation / genetics
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms / diagnosis*
Lung Neoplasms / genetics
Lung Neoplasms / pathology
Lung Neoplasms / surgery*
Male
Middle Aged
Neoplasm Metastasis
Neoplastic Stem Cells / metabolism
Neoplastic Stem Cells / pathology
Neoplastic Stem Cells / physiology*
Postoperative Period
Prognosis
RNA, Long Noncoding / physiology*
Small Cell Lung Carcinoma / diagnosis*
Small Cell Lung Carcinoma / genetics
Small Cell Lung Carcinoma / pathology
Small Cell Lung Carcinoma / surgery*
Survival Analysis
Transforming Growth Factor beta1 / genetics*
Treatment Outcome
Up-Regulation / genetics

Substances

Biomarkers, Tumor
RNA, Long Noncoding
TGFB1 protein, human
Transforming Growth Factor beta1
long noncoding RNA CASC11, human