The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research

Saumya Das; Extracellular RNA Communication Consortium; K Mark Ansel; Markus Bitzer; Xandra O Breakefield; Alain Charest; David J Galas; Mark B Gerstein; Mihir Gupta; Aleksandar Milosavljevic; Michael T McManus; Tushar Patel; Robert L Raffai; Joel Rozowsky; Matthew E Roth; Julie A Saugstad; Kendall Van Keuren-Jensen; Alissa M Weaver; Louise C Laurent

doi:10.1016/j.cell.2019.03.023

The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research

Cell. 2019 Apr 4;177(2):231-242. doi: 10.1016/j.cell.2019.03.023.

Authors

Saumya Das¹; Extracellular RNA Communication Consortium²; K Mark Ansel³, Markus Bitzer⁴, Xandra O Breakefield⁵, Alain Charest⁶, David J Galas⁷, Mark B Gerstein⁸, Mihir Gupta⁹, Aleksandar Milosavljevic¹⁰, Michael T McManus¹¹, Tushar Patel¹², Robert L Raffai¹³, Joel Rozowsky¹⁴, Matthew E Roth¹⁰, Julie A Saugstad¹⁵, Kendall Van Keuren-Jensen¹⁶, Alissa M Weaver¹⁷, Louise C Laurent¹⁸

Collaborators

Extracellular RNA Communication Consortium:
Asim B Abdel-Mageed, Catherine Adamidi, P David Adelson, Kemal M Akat, Eric Alsop, K Mark Ansel, Jorge Arango, Neil Aronin, Seda Kilinc Avsaroglu, Azadeh Azizian, Leonora Balaj, Iddo Z Ben-Dov, Karl Bertram, Markus Bitzer, Robert Blelloch, Kimberly A Bogardus, Xandra Owens Breakefield, George A Calin, Bob S Carter, Al Charest, Clark C Chen, Tanuja Chitnis, Robert J Coffey, Amanda Courtright-Lim, Saumya Das, Amrita Datta, Peter DeHoff, Thomas G Diacovo, David J Erle, Alton Etheridge, Marc Ferrer, Jeffrey L Franklin, Jane E Freedman, David J Galas, Timur Galeev, Roopali Gandhi, Aitor Garcia, Mark Bender Gerstein, Vikas Ghai, Ionita Calin Ghiran, Maria D Giraldez, Andrei Goga, Tasos Gogakos, Beatrice Goilav, Stephen J Gould, Peixuan Guo, Mihir Gupta, Fred Hochberg, Bo Huang, Matt Huentelman, Craig Hunter, Elizabeth Hutchins, Andrew R Jackson, M Yashar S Kalani, Pinar Kanlikilicer, Reka Agnes Karaszti, Kendall Van Keuren-Jensen, Anastasia Khvorova, Yong Kim, Hogyoung Kim, Taek Kyun Kim, Robert Kitchen, Richard P Kraig, Anna M Krichevsky, Raymond Y Kwong, Louise C Laurent, Minyoung Lee, Noelle L'Etoile, Shawn E Levy, Feng Li, Jenny Li, Xin Li, Gabriel Lopez-Berestein, Rocco Lucero, Bogdan Mateescu, A C Matin, Klaas E A Max, Michael T McManus, Thorsten R Mempel, Cindy Meyer, Aleksandar Milosavljevic, Debasis Mondal, Kenneth Jay Mukamal, Oscar D Murillo, Thangamani Muthukumar, Deborah A Nickerson, Christopher J O'Donnell, Dinshaw J Patel, Tushar Patel, James G Patton, Anu Paul, Elaine R Peskind, Mitch A Phelps, Chaim Putterman, Peter J Quesenberry, Joseph F Quinn, Robert L Raffai, Saritha Ranabothu, Shannon Jiang Rao, Cristian Rodriguez-Aguayo, Anthony Rosenzweig, Matthew E Roth, Joel Rozowsky, Marc S Sabatine, Nikita A Sakhanenko, Julie Anne Saugstad, Thomas D Schmittgen, Neethu Shah, Ravi Shah, Kerby Shedden, Jian Shi, Anil K Sood, Anuoluwapo Sopeyin, Ryan M Spengler, Robert Spetzler, Srimeenakshi Srinivasan, Sai Lakshmi Subramanian, Manikkam Suthanthiran, Kahraman Tanriverdi, Yun Teng, Muneesh Tewari, William Thistlethwaite, Thomas Tuschl, Karolina Kaczor Urbanowicz, Kasey C Vickers, Olivier Voinnet, Kai Wang, Alissa M Weaver, Zhiyun Wei, Howard L Weiner, Zachary R Weiss, Zev Williams, David T W Wong, Prescott G Woodruff, Xinshu Xiao, Irene K Yan, Ashish Yeri, Bing Zhang, Huang-Ge Zhang

Affiliations

¹ Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USA.
² http://exRNA.org/.
³ Department Microbiology & Immunology and Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA 94143, USA.
⁴ Department of Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
⁵ Department of Neurology and Center for Molecular Imaging Research Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129 USA; Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA.
⁶ Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
⁷ Pacific Northwest Research Institute, Seattle, WA 98122, USA.
⁸ Program in Computational Biology & Bioinformatics, Department of Molecular Biophysics & Biochemistry, Department of Computer Science, and Department of Statistics & Data Science, Yale University, New Haven, CT 06520, USA.
⁹ Department of Neurosurgery, University of California, San Diego, CA 92093, USA.
¹⁰ Molecular and Human Genetics Department, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA; Program in Quantitative and Computational Biosciences, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA.
¹¹ Department of Microbiology and Immunology, University of California San Francisco Diabetes Center, WM Keck Center for Noncoding RNAs, University of California, San Francisco, San Francisco, CA 94143, USA.
¹² Department of Transplantation, Mayo Clinic, Jacksonville, FL 32224, USA.
¹³ Department of Surgery, University of California San Francisco & Veterans Affairs Medical Center San Francisco, San Francisco, CA 94121, USA.
¹⁴ Department of Molecular Biophysics and Biochemistry, Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT 06520, USA.
¹⁵ Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, OR 97239, USA.
¹⁶ Neurogenomics, Translational Genomics Research Institute, Phoenix, AZ 85284, USA.
¹⁷ Department of Cell and Developmental Biology, Vanderbilt University School of Medicine and Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
¹⁸ Department of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: llaurent@ucsd.edu.

Abstract

The Extracellular RNA Communication Consortium (ERCC) was launched to accelerate progress in the new field of extracellular RNA (exRNA) biology and to establish whether exRNAs and their carriers, including extracellular vesicles (EVs), can mediate intercellular communication and be utilized for clinical applications. Phase 1 of the ERCC focused on exRNA/EV biogenesis and function, discovery of exRNA biomarkers, development of exRNA/EV-based therapeutics, and construction of a robust set of reference exRNA profiles for a variety of biofluids. Here, we present progress by ERCC investigators in these areas, and we discuss collaborative projects directed at development of robust methods for EV/exRNA isolation and analysis and tools for sharing and computational analysis of exRNA profiling data.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Biomarkers
Cell-Free Nucleic Acids / genetics*
Cell-Free Nucleic Acids / metabolism*
Extracellular Vesicles / genetics*
Humans
Knowledge Bases
MicroRNAs / genetics
RNA / genetics

Substances

Biomarkers
Cell-Free Nucleic Acids
MicroRNAs
RNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding