High separation efficiency of gold nanomaterials of different aspect ratio and size using capillary transient isotachophoresis

Kathryn R Riley; Hind El Hadri; Jiaojie Tan; Vincent A Hackley; William A MacCrehan

doi:10.1016/j.chroma.2019.03.054

High separation efficiency of gold nanomaterials of different aspect ratio and size using capillary transient isotachophoresis

J Chromatogr A. 2019 Aug 2:1598:216-222. doi: 10.1016/j.chroma.2019.03.054. Epub 2019 Mar 25.

Authors

Kathryn R Riley¹, Hind El Hadri², Jiaojie Tan², Vincent A Hackley², William A MacCrehan³

Affiliations

¹ National Institute of Standards and Technology, Material Measurement Laboratory - Chemical Sciences Division, 100 Bureau Drive, Gaithersburg, MD, 20899, USA. Electronic address: kriley1@swarthmore.edu.
² National Institute of Standards and Technology, Material Measurement Laboratory - Materials Measurement Science Division, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
³ National Institute of Standards and Technology, Material Measurement Laboratory - Chemical Sciences Division, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.

PMID: 30948041
DOI: 10.1016/j.chroma.2019.03.054

Abstract

Two modes of capillary electrophoresis (CE), capillary zone electrophoresis (CZE) and capillary transient isotachophoresis (ctITP), were compared for the detection and separation of spherical gold nanoparticles (AuNPs) and gold nanorods (AuNRs). The development of ctITP using two different leading ions is described. Overall, when compared to traditional capillary zone electrophoresis (CZE), ctITP resulted in improved peak shape and peak efficiency. Specifically, the number of theoretical plates for AuNR samples increased by a factor of 2-2.5 depending on the choice of leading ion. Further, using ctITP two AuNRs differing by aspect ratio were baseline resolved, whereas the same AuNRs could not be separated using CZE or other techniques like single particle inductively coupled plasma mass spectrometry (spICP-MS) and asymmetric flow field-flow fractionation (AF4). The results of this study demonstrate that ctITP is an efficient on-line technique for the improved detection and separation of gold nanomaterials in CE.

Keywords: Capillary transient isotachophoresis; Flow field flow fractionation; Gold nanoparticles; Gold nanorods; Single particle inductively coupled plasma mass spectrometry.

MeSH terms

Chemistry Techniques, Analytical / methods*
Electrolytes
Electrophoresis, Capillary
Gold / chemistry*
Gold / isolation & purification*
Isotachophoresis*
Metal Nanoparticles / chemistry*
Nanotubes / chemistry*
Particle Size

Substances

Electrolytes
Gold