Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H2 O2 -Dependent Biocatalytic Oxidation Reactions

Angew Chem Int Ed Engl. 2019 Jun 3;58(23):7873-7877. doi: 10.1002/anie.201902380. Epub 2019 Apr 30.

Abstract

An increasing number of biocatalytic oxidation reactions rely on H2 O2 as a clean oxidant. The poor robustness of most enzymes towards H2 O2 , however, necessitates more efficient systems for in situ H2 O2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H2 O2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

Keywords: biocatalysis; formate oxidase; hydrogen peroxide; oxidation; oxyfunctionalisation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus oryzae / enzymology*
  • Biocatalysis
  • Formates / metabolism*
  • Fungal Proteins / metabolism*
  • Hydrogen Peroxide / metabolism*
  • Kinetics
  • Mixed Function Oxygenases / metabolism*
  • Oxidation-Reduction
  • Oxidoreductases / metabolism*
  • Oxygen / metabolism*

Substances

  • Formates
  • Fungal Proteins
  • Hydrogen Peroxide
  • Mixed Function Oxygenases
  • Oxidoreductases
  • peroxygenase
  • Oxygen