Abstract
In this study, an extract from the bulbs of Cyrtanthus contractus showed strong anti-inflammatory activity in vitro. The extract was partially separated into 14 fractions and analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry metabolomics, and the correlation coefficients were calculated between biological activities and metabolite levels. As a result, the top-scoring metabolite narciclasine (1) is proposed as the active principle of C. contractus. This was confirmed by comparing the biological effect of crude extract with that of an authentic standard.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amaryllidaceae / chemistry*
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Amaryllidaceae Alkaloids / chemistry
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Amaryllidaceae Alkaloids / pharmacology*
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Cell Adhesion / drug effects
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Cell Line
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Cell Survival / drug effects
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Chromatography, High Pressure Liquid
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Human Umbilical Vein Endothelial Cells / drug effects
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Humans
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Intercellular Adhesion Molecule-1 / drug effects
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Intercellular Adhesion Molecule-1 / metabolism
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Mass Spectrometry
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Metabolomics*
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Phenanthridines / chemistry
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Phenanthridines / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Roots / chemistry
Substances
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Amaryllidaceae Alkaloids
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Anti-Inflammatory Agents, Non-Steroidal
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Phenanthridines
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Plant Extracts
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Intercellular Adhesion Molecule-1
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narciclasine