Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC

Ibrahim Q Saeed; Niklaas J Buurma

doi:10.1007/978-1-4939-9179-2_13

Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC

Methods Mol Biol. 2019:1964:169-183. doi: 10.1007/978-1-4939-9179-2_13.

Authors

Ibrahim Q Saeed¹, Niklaas J Buurma²

Affiliations

¹ Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region, Iraq.
² Physical Organic Chemistry Centre, School of Chemistry, Cardiff University, Cardiff, UK. Buurma@cardiff.ac.uk.

PMID: 30929243
DOI: 10.1007/978-1-4939-9179-2_13

Abstract

I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.

Keywords: Complex equilibrium systems; Confidence interval; Data analysis; I2CITC; ITC; Isothermal titration calorimetry; Thermodynamics.

MeSH terms

Binding Sites
Calorimetry / methods*
Ligands
Protein Binding
Thermodynamics*

Substances

Ligands