Protein Expression Analysis by Western Blot and Protein-Protein Interactions

Methods Mol Biol. 2019:1968:101-111. doi: 10.1007/978-1-4939-9199-0_9.

Abstract

Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.

Keywords: Monoclonal antibody; Pneumolysin; PpmA; Urine; Western-blot.

MeSH terms

  • Antibodies, Bacterial / metabolism
  • Antibodies, Monoclonal / metabolism
  • Bacterial Proteins / urine
  • Blotting, Western
  • Humans
  • Pneumococcal Infections / diagnosis
  • Pneumococcal Infections / metabolism*
  • Pneumococcal Infections / urine*
  • Streptococcus pneumoniae / immunology
  • Streptococcus pneumoniae / metabolism
  • Streptolysins / urine

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Bacterial Proteins
  • PpmA protein, Streptococcus pneumoniae
  • Streptolysins
  • plY protein, Streptococcus pneumoniae