High antioxidant capability interacts with respiration to mediate two Alexandrium species growth exploitation of photoperiods and light intensities

Abstract

Light drives phytoplankton photosynthesis, so phytoplankton in their living habitats must exploit variable light levels and exposure durations, depending upon seasons, latitudes, depths and mixing events. Comparative growth, physiology and biochemical compositions were explored for the small Alexnadrium minutum (˜40 μm³ biovolume) and large Alexandrium catenella (˜9300 μm³ biovolume), globally wide spread coastal toxic red tide dinoflagellates, responding to a matrix of photoperiods (Light:Dark, 8:16, 16:8 and 24:0) and growth light irradiances. Smaller A. minutum grew faster under shorter photoperiods across growth light levels, while larger A. catenella grew fastest under longer photoperiods at the lowest applied light level. Photosystem II function responded largely to the instantaneous growth light level across photoperiod lengths, while the cell biovolume-based respiration, antioxidant capacity as well as cell composition responded more to photoperiod duration than to light level. These complex photophysiological responses resolved into linear correlations between growth rate versus cellular antioxidant activity and versus dark respiration, indicating that respiration energizes cellular antioxidant systems to benefit the growth of the cells. These results show the growth responses of Alexandrium species to light levels across photoperiods vary with species, and possibly with cell size. Together with previous results this puts a note of caution on meta-analytical extrapolations of physiological responses to light intensity derived from studies applying different photoperiods to different taxa, because different taxa show differential, even opposite growth responses to photoperiods and light intensities.

Keywords: Alexandrium; Antioxidant activity; Cell size; Dinoflagellate; Growth; Photoperiod; RUBISCO; Respiration.