Whole-genome sequencing of Escherichia coli isolated from contaminated meat samples collected from the Northern Region of Ghana reveals the presence of multiple antimicrobial resistance genes

Abstract

Objectives: This study reports the draft genomes of 14 Escherichia coli isolated from contaminated meat samples collected from the Northern Region of Ghana in order to determine the presence of antimicrobial resistance genes (ARGs) and genetic relatedness of the isolates.

Methods: The 14 E. coli isolates were of beef (n=3), mutton (n=2), chevon (n=3), local chicken (n=3) and guinea fowl (n=3) origin. Whole-genome sequencing (WGS) was performed using an Illumina MiSeq sequencer. Double-disk synergy test (DDST) was also used to confirm the production of extended-spectrum β-lactamase (ESBL).

Results: WGS confirmed the identity of all of the E. coli isolates. All of the isolates contained at least one ARG and 57.1% (8/14) of them were multidrug-resistant (MDR). The mdf(A) gene was most common ARG, found in all 14 isolates. DDST confirmed the production of an ESBL in a MDR E. coli of guinea fowl origin. The sequence types (STs) varied among the E. coli isolates, with the exception of three isolates of ST155. Similarly, the serotypes of the E. coli isolates from meat sample were genetically diverse. Eleven different plasmid sequences were detected in ten of the isolates.

Conclusion: E. coli from contaminated meat sources in Ghana possessed multiple ARGs and were genetically diverse. To the best of our knowledge, this is the first work on WGS of E. coli isolated from various meat samples in the study area. The sequence data add to data base for epidemiological studies.

Keywords: Antimicrobial resistance; Escherichia coli; Foodborne pathogen; Genetic relatedness; Meat; Whole-genome sequencing.