Amphiphilic carboxylated cellulose-g-poly(l-lactide) copolymer nanoparticles for oleanolic acid delivery

Yingsa Wang; Pengbo Zhu; Guiliang Li; Shangbin Zhu; Kefeng Liu; Yanxue Liu; Jing He; Jiandu Lei

doi:10.1016/j.carbpol.2019.03.033

Amphiphilic carboxylated cellulose-g-poly(l-lactide) copolymer nanoparticles for oleanolic acid delivery

Carbohydr Polym. 2019 Jun 15:214:100-109. doi: 10.1016/j.carbpol.2019.03.033. Epub 2019 Mar 14.

Authors

Yingsa Wang¹, Pengbo Zhu¹, Guiliang Li¹, Shangbin Zhu¹, Kefeng Liu¹, Yanxue Liu¹, Jing He², Jiandu Lei¹

Affiliations

¹ Beijing Key Laboratory of Lignocellulosic Chemistry, MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China.
² Beijing Key Laboratory of Lignocellulosic Chemistry, MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. Electronic address: hejing2008@sina.com.

PMID: 30925977
DOI: 10.1016/j.carbpol.2019.03.033

Abstract

Carboxylated cellulose nanocrystals (CCNs), as one of nanocellulose are promising hydrophilic biomass materials for drug delivery. In this work, a series of amphiphilic carboxylated cellulose-graft-Poly(L-lactide) (CC-g-PLLA) copolymers were synthesized via ring-opening polymerization (ROP) method. The copolymers were characterized by ¹H-NMR, FT-IR, WXRD and TGA, and their solubility in organic solvents was improved. Then, these amphiphilic copolymers were self-assembled into nanoparticles for delivery of anticancer drug oleanolic acid (OA). The copolymer (DS_PLLA 2.03) nanoparticles displayed the smallest size (196.82 ± 9.14 nm) and the highest drug loading efficiency (24.76 ± 0.58%). The nanoparticles exhibited a spherical shape, well water solubility of OA (16.9 mg/mL) and a prolonged drug release (120 h). In vitro and In vivo study indicated that the nanoparticles maintained cytotoxicity to 4T1 cells and MCF-7 cells and displayed high antitumor efficiency. The amphiphilic CC-g-PLLA copolymer nanoparticles provide a novel platform for drug delivery.

Keywords: Amphiphilic copolymer; Carboxylated cellulose nanocrystals; Drug delivery; Nanoparticles; Oleanolic acid.