Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis

Cell Rep. 2019 Mar 26;26(13):3657-3671.e5. doi: 10.1016/j.celrep.2019.02.106.

Abstract

Aurora kinase B (AURKB), a central regulator of chromosome segregation and cytokinesis, is aberrantly expressed in various cancer cells. However, the relationship of AURKB and oncogenic viruses in cancer progression remains unclear. Here, we reveal that N-cleaved isoforms of AURKB exist in several oncovirus-associated tumor cells and patient cancer tissues, including Kaposi's sarcoma-associated herpesvirus (KSHV), Epstein-Barr virus (EBV), and human papillomavirus virus (HPV). Mechanistically, in KSHV-infected tumor cells, the latent viral antigen LANA cleaves AURKB at Asp76 in a serine protease-dependent manner. The N'-AURKB relocalizes to the spindle pole and promotes the metaphase-to-telophase transition in mitotic cells. Introduction of N'-AURKB but not C'-AURKB promotes colony formation and malignant growth of tumor cells in vitro and in vivo using a murine xenograft model. Altogether, our findings uncover a proteolytic cleavage mechanism by which oncoviruses induce cancer cell segregation and tumorigenesis.

Keywords: AURKB; EBV; HPV; KSHV; cell segregation; oncovirus; protein cleavage; tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Aurora Kinase B / metabolism*
  • Carcinogenesis*
  • Chromosome Segregation
  • Female
  • HEK293 Cells
  • HeLa Cells
  • Herpesvirus 8, Human / physiology
  • Humans
  • Male
  • Mice
  • Mice, Inbred NOD
  • Middle Aged
  • Mitosis
  • Neoplasms / metabolism
  • Neoplasms / virology*
  • Oncogenic Viruses / physiology*
  • Xenograft Model Antitumor Assays
  • Young Adult

Substances

  • AURKB protein, human
  • Aurora Kinase B