Humin (HM) is a complex mixture of molecules produced in the different biological processes, and the structural evolution of HM in the agricultural wastes composting are not well-known. Elucidating and comparing the structural evolution during livestock manure (LMC) and straw wastes (SWC) composting can help one to better understand the fates, features, and environmental impacts of HM. This study exploits excitation emission matrix-parallel factor (EEM-PARAFAC), two-dimensional correlation spectroscopy (2D-CoS), hetero-2DCoS, and structural equation model (SEM) to compare the fate of the HM. We fit a three-component EEM-PARAFAC model to characterize HM extracted from LMC and SWC. The results show that the HM evolution has a significant difference between LMC and SWC. As a result, the opposite change tendency and different change order of HM fluorescent components determine the different synthesis formation and evolution mechanisms. The diverse organic matter composition and dominant microbes might be the reason for the different evolution mechanism. Based on these results, a comprehensive view of the component changes of HM in the composting process is obtained. Furthermore, the superior potential of such an integrated approach during investigating the complex evolution in the environment was also demonstrated.
Keywords: 2D-COS; EEM-PARAFAC; SEM; humin; livestock manure composting; straw waste composting.