Tridimensional alginate disks of tunable topologies for mammalian cell encapsulation

Alejandra Suarez-Arnedo; Diana M Narváez; Paula Sarmiento; Laura Bocanegra; Felipe Salcedo; Carolina Muñoz-Camargo; Helena Groot; Juan C Cruz

doi:10.1016/j.ab.2019.03.008

Tridimensional alginate disks of tunable topologies for mammalian cell encapsulation

Anal Biochem. 2019 Jun 1:574:31-33. doi: 10.1016/j.ab.2019.03.008. Epub 2019 Mar 21.

Authors

Alejandra Suarez-Arnedo¹, Diana M Narváez¹, Paula Sarmiento², Laura Bocanegra¹, Felipe Salcedo², Carolina Muñoz-Camargo³, Helena Groot¹, Juan C Cruz⁴

Affiliations

¹ Human Genetics Laboratory, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
² Department of Chemical Engineering, Universidad de los Andes, Bogotá, Colombia.
³ Department of Biomedical Engineering, Universidad de los Andes, Bogotá, Colombia.
⁴ Department of Biomedical Engineering, Universidad de los Andes, Bogotá, Colombia. Electronic address: jc.cruz@uniandes.edu.co.

PMID: 30905690
DOI: 10.1016/j.ab.2019.03.008

Abstract

We have developed a protocol to produce three-dimensional matrices based on alginate hydrogels for mammalian cell encapsulation. Based on the gelation properties of this polysaccharide, we implemented a calcium ion-based diffusion method where the designed hydrogels can be obtained with well-defined mechanical properties and replicable 3D topologies. The developed protocol can be extended to different types of alginates and an ample range of concentrations. This makes it very attractive for various biomedical applications where strict control over structure-function relationships is desirable.

Keywords: Alginate; Encapsulation; Hydrogels; Mammalian cells.

MeSH terms

Alginates / chemistry*
Animals
Biocompatible Materials
Cell Encapsulation*
Hydrogels
Mammals
Structure-Activity Relationship

Substances

Alginates
Biocompatible Materials
Hydrogels