Identification and expression analysis of 19 CC chemokine genes in orange-spotted grouper (Epinephelus coioides)

Jiann-Horng Leu; Chi-Hang Tsai; Jyh-Ming Tsai; Chia-Hsun Yang; Chih-Yuan Hsueh; Hsin-Yiu Chou

doi:10.1016/j.dci.2019.03.006

Identification and expression analysis of 19 CC chemokine genes in orange-spotted grouper (Epinephelus coioides)

Dev Comp Immunol. 2019 Aug:97:1-10. doi: 10.1016/j.dci.2019.03.006. Epub 2019 Mar 20.

Authors

Jiann-Horng Leu¹, Chi-Hang Tsai², Jyh-Ming Tsai³, Chia-Hsun Yang², Chih-Yuan Hsueh⁴, Hsin-Yiu Chou⁵

Affiliations

¹ Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan, ROC; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, ROC. Electronic address: jiholeu@ntou.edu.tw.
² Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan, ROC.
³ Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan, ROC.
⁴ Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, ROC.
⁵ Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, ROC; Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan, ROC.

PMID: 30904428
DOI: 10.1016/j.dci.2019.03.006

Abstract

In this study, we describe 19 different CC chemokine genes from the orange-spotted grouper, Epinephelus coioides, identified by the analysis of the spleen transcriptome. Multiple sequence alignment of the 19 CC chemokines showed that although two genes, EcSCYA115 and EcSCYA117, shared 80% amino acid similarity (72% identity), the majority exhibited low similarity to each other. Phylogenetic analysis divided the 19 CC chemokines into six major groups. Tissue distribution analysis by RT-PCR showed that most of these chemokines were ubiquitously expressed in the 9 examined tissues, whereas some exhibited tissue-preferential expression patterns. For example, EcSCYA103 was preferentially expressed in fin and gill; EcSCYA109 in head kidney and spleen; EcSCYA114 in fin, gill, and liver; and EcSCYA119 in fin and stomach. Quantitative RT-PCR showed that after challenge with grouper iridovirus (GIV), four of the 19 CC chemokine genes, EcSYCA102, EcSYCA103, EcSYCA116, and EcSYCA118, were highly induced in the spleen. The expression of these four genes could also be upregulated by LPS and poly (I:C) challenges, suggesting that these four genes might be involved in immune response against invading pathogens.

Keywords: CC chemokine; Epinephelus coioides; GIV; Grouper iridovirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animal Fins / metabolism
Animals
Bass / genetics*
Bass / virology
Chemokines, CC / classification
Chemokines, CC / genetics*
Fish Proteins / genetics*
Gene Expression Profiling / methods
Gills / metabolism
Host-Pathogen Interactions
Iridovirus / physiology
Multigene Family*
Organ Specificity / genetics
Sequence Homology, Amino Acid
Spleen / metabolism*
Transcriptome / genetics*

Substances

Chemokines, CC
Fish Proteins