Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

Xuemei Xu; Shiqi Fang; Yuan Zhuang; Shanshan Wu; Qingling Pan; Longjie Li; Xiaohui Wang; Xueqing Sun; Bifeng Liu; Yuzhou Wu

doi:10.3390/ma12060949

Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

Materials (Basel). 2019 Mar 21;12(6):949. doi: 10.3390/ma12060949.

Authors

Xuemei Xu¹, Shiqi Fang², Yuan Zhuang³, Shanshan Wu⁴, Qingling Pan⁵, Longjie Li⁶, Xiaohui Wang⁷, Xueqing Sun⁸, Bifeng Liu⁹, Yuzhou Wu^{10

11}

Affiliations

¹ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. xuxuemei@hust.edu.cn.
² Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. fangshiqi@hust.edu.cn.
³ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. zhuangyuan@hust.edu.cn.
⁴ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. wushanshan@hust.edu.cn.
⁵ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. panqingling@hust.edu.cn.
⁶ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. lilongjie18@hust.edu.cn.
⁷ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. m201670275@hust.edu.cn.
⁸ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. sunxueqing@hust.edu.cn.
⁹ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. bfliu@hust.edu.cn.
¹⁰ Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. wuyuzhou@hust.edu.cn.
¹¹ Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany. wuyuzhou@hust.edu.cn.

Abstract

DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications.

Keywords: DNA origami; cationic albumin; cellular uptake; stability.

Abstract

Grants and funding