Generation and characterization of a human induced pluripotent stem cell (iPSC) line (HEBHMUi001-A) from a sporadic Parkinson's disease patient

Jun Ma; Ruiyun Guo; Yongzhou Song; Jing Zhang; Baofeng Feng; Asiamah Ernest Amponsah; Desheng Kong; Jingjing He; Wei Zhang; Aijing Liu; Lin Wei; Sanbing Shen; Timothy O'Brien; Huixian Cui

doi:10.1016/j.scr.2019.101417

Generation and characterization of a human induced pluripotent stem cell (iPSC) line (HEBHMUi001-A) from a sporadic Parkinson's disease patient

Stem Cell Res. 2019 Apr:36:101417. doi: 10.1016/j.scr.2019.101417. Epub 2019 Mar 7.

Authors

Jun Ma¹, Ruiyun Guo², Yongzhou Song³, Jing Zhang⁴, Baofeng Feng², Asiamah Ernest Amponsah², Desheng Kong², Jingjing He², Wei Zhang², Aijing Liu³, Lin Wei², Sanbing Shen⁵, Timothy O'Brien⁵, Huixian Cui⁶

Affiliations

¹ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China.
² Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China.
³ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Second Affiliated Hospital of Hebei Medical University, China.
⁴ The Fourth Hospital of Shijiazhuang, China.
⁵ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland.
⁶ Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China. Electronic address: Huixiancuihmu@163.com.

PMID: 30875588
DOI: 10.1016/j.scr.2019.101417

Abstract

We generated a human induced pluripotent stem cell (iPSC) line from the skin fibroblasts of a 62-year-old female patient clinically diagnosed with sporadic Parkinson's disease (PD). The generated iPSCs maintained their normal karyotype, expressed pluripotency stem cell markers, and were demonstrated to be capable of differentiating into cells representative of the three embryonic germ layers. The generated line could be used for PD modeling in order to understand the mechanisms that influence the disorder.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line*
Female
Fibroblasts
Genetic Markers
Humans
Induced Pluripotent Stem Cells*
Karyotype
Middle Aged
Parkinson Disease / genetics*
Skin

Substances

Genetic Markers