Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes

Carlos André Ornelas Ricart; Rayner Myr Lauterjung Queiroz; Agenor de Castro Moreira Dos Santos Júnior; Sébastien Charneau

doi:10.1007/978-1-4939-9148-8_6

Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes

Methods Mol Biol. 2019:1955:77-87. doi: 10.1007/978-1-4939-9148-8_6.

Authors

Carlos André Ornelas Ricart¹, Rayner Myr Lauterjung Queiroz², Agenor de Castro Moreira Dos Santos Júnior³, Sébastien Charneau³

Affiliations

¹ Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry, University of Brasilia, Brasília, DF, Brazil. ricart@unb.br.
² Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK.
³ Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry, University of Brasilia, Brasília, DF, Brazil.

PMID: 30868520
DOI: 10.1007/978-1-4939-9148-8_6

Abstract

The preparation of subproteome fractions prior to proteome analysis provides both the enrichment of proteins sub-represented in global proteome analysis and information on the cellular localization of the identified proteins. Here we describe protocols for the preparation of Trypanosoma cruzi surface and extracellular and nuclear fractions for further subproteome analysis.

Keywords: Biotinylation; Cell surface shaving; Nucleus; Plasma membrane; Proteome; Secretome.

MeSH terms

Biotinylation
Cell Fractionation / methods*
Cell Membrane / chemistry
Cell Nucleus / chemistry
Chemical Precipitation
Chromatography, Affinity / methods
Chromatography, Liquid / methods
Humans
Proteome / analysis*
Proteomics / methods*
Protozoan Proteins / analysis*
Tandem Mass Spectrometry / methods*
Trypanosoma cruzi / chemistry*
Ultracentrifugation / methods

Substances

Proteome
Protozoan Proteins