Textile industrial wastewater samples were taken from the Panki site 5 industrial area of Kanpur city, India. Atomic Absorption spectrophotometer and Gas Chromatography-Mass spectrometry techniques have shown that the wastewater contained several heavy metals and organic pollutants (Khan and Malik, 2017) [1]. Further, in order to explore the potential toxicity of these pollutants present in the effluent, a battery of short-term biological assays (Ames test, DNA repair defective mutation assay and Allium cepa chromosomal aberration test) were used. Wastewater samples were concentrated with XAD-4/8 resins and liquid-liquid extraction procedure. XAD-concentrated samples were more mutagenic than the liquid-liquid extracted samples. Ames TA98 and polA (SOS defective) strains were the most responsive strains. The wastewater also resulted in significant decline in mitotic index and induced chromosomal aberrations in A. cepa roots. The findings thus showed that the combination of physico-chemical analysis alongwith the toxicity assessment (using short term biological assays) would provide valuable and more realistic information about the joint toxicity of chemical pollutants present in the textile effluent.
Keywords: Allium cepa; Ames test; Genotoxicity; Mitotic index; Mutagenicity; Textile industry.