A chiral assembly of gold nanoparticle trimer-based biosensors for ultrasensitive detection of the major allergen tropomyosin in shellfish

Yanbo Wang; Zhiheng Rao; Jinru Zhou; Lei Zheng; Linglin Fu

doi:10.1016/j.bios.2019.02.038

A chiral assembly of gold nanoparticle trimer-based biosensors for ultrasensitive detection of the major allergen tropomyosin in shellfish

Biosens Bioelectron. 2019 May 1:132:84-89. doi: 10.1016/j.bios.2019.02.038. Epub 2019 Feb 21.

Authors

Yanbo Wang¹, Zhiheng Rao¹, Jinru Zhou¹, Lei Zheng², Linglin Fu³

Affiliations

¹ Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
² School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
³ Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China. Electronic address: fulinglin@zjgsu.edu.cn.

PMID: 30856431
DOI: 10.1016/j.bios.2019.02.038

Abstract

A biosensor based on a chiral assembly of polymer of gold nanoparticle (AuNP) trimers was developed for the detection and quantification of the major shellfish allergen tropomyosin (TROP). TROP and anti-TROP monoclonal antibodies (mAb) were immobilized on 20 nm and 30 nm 16-mercaptohexadecanoic acid (16-MHDA) functionalized AuNPs to assemble a trimer, which has a Circular dichroism (CD) signal. The free TROP from samples was quantified as an inhibitor for the formation of the AuNP trimer. The AuNP trimer-based biosensor allowed for the selective determination of TROP in the range of 0.1-15 ng mL^-1 with the limit of detection (LOD) of 21 pg mL^-1 (S/N = 3) and the limit of quantitation (LOQ) of 70 pg mL^-1 (S/N = 10). The use of a AuNP trimer-based biosensor with simple sample preparation functions with specificity and accuracy; this highlights its applicability for the detection of allergens in shellfish products, related products and their production lines. Furthermore, based on the less conserved sequences of TROP in phylogenetically different species, this biosensor is currently being used to identify the adulteration of shellfish products using TROP as biomarker.

Keywords: Circular dichroism spectroscopy; Gold nanoparticle; Tropomyosin.

Publication types

Evaluation Study

MeSH terms

Allergens / analysis*
Antibodies, Immobilized / chemistry
Biosensing Techniques / methods*
Dimerization
Food Analysis / methods
Gold / chemistry*
Immunoassay / methods
Limit of Detection
Metal Nanoparticles / chemistry*
Shellfish / analysis*
Shellfish Proteins / analysis*
Tropomyosin / analysis*

Substances

Allergens
Antibodies, Immobilized
Shellfish Proteins
Tropomyosin
Gold