Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production

Jong-Hun Park; Jeong-Hoon Park; Young-Bo Sim; Sang-Hyoun Kim; Hee-Deung Park

doi:10.1016/j.biortech.2019.02.124

Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production

Bioresour Technol. 2019 Jun:282:63-68. doi: 10.1016/j.biortech.2019.02.124. Epub 2019 Mar 1.

Authors

Jong-Hun Park¹, Jeong-Hoon Park², Young-Bo Sim³, Sang-Hyoun Kim³, Hee-Deung Park⁴

Affiliations

¹ School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, South Korea.
² School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, South Korea; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208, USA.
³ School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
⁴ School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, South Korea; KU-KIST Green School, Graduate School of Energy and Environment, Korea University, Seoul 02841, South Korea. Electronic address: heedeung@korea.ac.kr.

PMID: 30851575
DOI: 10.1016/j.biortech.2019.02.124

Abstract

This study aimed to investigate the relationship among dynamic membrane (DM) formation, metabolic flux, and microbial community population in dark fermentative hydrogen production. A continuously stirred tank reactor was equipped with an external submerged polyester screen mesh and inoculated with heat-treated anaerobic sludge without immobilization. DM was successfully developed on the polyester mesh and provided high-rate hydrogen production at 60.5 L H₂/L.d and 2.39 mol H₂/mol glucose_added. DM formation was along with tightly bound extracellular polymeric substances. Flux balance analysis revealed that formation of DM altered the metabolic pathways for acetic acid production from homoacetogenesis to hydrogenesis. Bacterial community analysis suggested that Sporolactobacillaceae would contributed to this metabolic pathway shift. Nevertheless, lactic acid was not accumulated and assumed to be consumed by hydrogen producers including Clostridia.

Keywords: Biofilm; Dynamic membrane; Flux balance analysis; High-rate bio-H(2); Sporolactobacillaceae.

MeSH terms

Acetic Acid / metabolism
Clostridium / metabolism
Fermentation*
Hydrogen / metabolism*
Microbiota*
Sewage / microbiology

Substances

Sewage
Hydrogen
Acetic Acid