Effect of bovine serum albumin on stability and transport of kaolinite colloid

Abstract

The stability and transport of clay colloids in groundwater are strongly influenced by colloid interactions with dissolved organic matter (DOM). Protein is an important DOM component that is ubiquitous in natural water, reclaimed water, and soil solutions. To date, the interactions between clay colloids and proteins have not been fully studied. The objective of this study was to examine the effect of bovine serum albumin (BSA), a representative protein, on the stability, aggregation, and transport of kaolinite colloids under neutral pH conditions. Hydrodynamic diameter and ζ-potential measurements, stability tests, and column transport experiments were performed in salt solutions with a range of ionic strengths and different BSA concentrations at pH 7. Additionally, BSA-kaolinite colloid interactions were studied using TEM and batch adsorption experiments. The experimental results showed that BSA prevented colloid aggregation and increased the stability and transport of colloids, especially at high ionic strength, even though the charges of kaolinite colloids were less negative in the presence of BSA. Theoretical calculation of the interaction energies indicated that XDLVO theory, in which the steric force is considered due to BSA adsorption, could correctly quantify the interaction energies in the presence of BSA. This study demonstrated that the role of protein needs to be determined in order to better predict the overall effect of DOM on particle aggregation and transport in the soil environment.

Keywords: BSA; Clay colloids; Fine particle aggregation and transport; Steric force; XDLVO theory.