Abstract
Here, we present an ultra-enhanced immunoassay for sensitive and reliable biomarker detection using layer-by-layer assembly and transcription-assisted light-up aptamer generation to induce signal amplification. This dendrimer structure-based transcription immunoassay is ∼1500 times more sensitive than commercial fluorescence ELISA, achieving a detection limit of 108 aM.
MeSH terms
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Antibodies / immunology
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Aptamers, Nucleotide / chemistry*
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Aptamers, Nucleotide / metabolism
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Biomarkers / analysis
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Dendrimers / chemistry
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Fluorescent Dyes / chemistry
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Immunoassay / methods*
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Limit of Detection
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Spectrometry, Fluorescence
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alpha-Fetoproteins / analysis
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alpha-Fetoproteins / immunology
Substances
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Antibodies
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Aptamers, Nucleotide
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Biomarkers
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Dendrimers
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Fluorescent Dyes
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alpha-Fetoproteins