Capillary Electrophoresis Analysis of Prostate-Specific Antigen (PSA)

Noemi Farina-Gomez; Diana Navarro-Calderon; Angel Puerta; Monica Gonzalez; José Carlos Diez-Masa; Mercedes de Frutos

doi:10.1007/978-1-4939-9213-3_16

Capillary Electrophoresis Analysis of Prostate-Specific Antigen (PSA)

Methods Mol Biol. 2019:1972:221-234. doi: 10.1007/978-1-4939-9213-3_16.

Authors

Noemi Farina-Gomez¹, Diana Navarro-Calderon¹, Angel Puerta¹, Monica Gonzalez¹, José Carlos Diez-Masa¹, Mercedes de Frutos²

Affiliations

¹ Institute of Organic Chemistry, (IQOG-CSIC), Madrid, Spain.
² Institute of Organic Chemistry, (IQOG-CSIC), Madrid, Spain. mfrutos@iqog.csic.es.

PMID: 30847795
DOI: 10.1007/978-1-4939-9213-3_16

Abstract

The Capillary Electrophoresis (CE) profile of isoforms (peaks) of a glycoprotein can be useful to show alterations in its posttranslational modifications (PTMs) linked to diseases. These changes can modify the electrophoretic mobility of these isoforms in a minor extent and, therefore, very reproducible CE methods are needed to detect them. In this chapter, a method for the analysis of prostate-specific antigen (PSA) by Capillary Zone Electrophoresis (CZE) with UV detection is detailed. High reproducibility in the separation of a large number of PSA isoforms is achieved by performing capillary conditioning in acid media and by using a background electrolyte (BGE) at pH 8.0 formulated with decamethonium bromide and urea.

Keywords: Cancer biomarker; Capillary conditioning; Glycoform; Isoform; PSA; Prostate-specific antigen; Proteoform; Reproducibility.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Capillary / methods*
Humans
Male
Prostate-Specific Antigen / analysis*
Protein Isoforms / analysis
Time Factors

Substances

Protein Isoforms
Prostate-Specific Antigen